Staining clinical specimens for acid-fast bacilli by means of a mechanical conveyor system.

نویسندگان

  • G V Heimer
  • N Joseph
  • C E Taylor
چکیده

A Cyto-Tek staining machine designed for Papanicolau staining of cervical smears has been adapted for auramine staining of smears of sputum and other clinical specimens for acid-fast bacilli. The results compared favourably with those obtained by a manual method of staining. Contamination of negative smears by acid-fast bacilli from positive smears was not found. Since this study the machine has continued to be in routine use with a considerable saving in labour.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of Polymerase Chain Reaction, Ziehl-Neelsen Staining and Histopathologic Findings in Formalin-fixed, Paraffin-Embedded Tissue Specimens for Diagnosis of Tuberculosis

Background and Objective: Tuberculosis is still a major health problem, involving about 1/3 of the world´s population. Diagnosis is difficult when we only use Ziehl-Neelson staining. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study was to compare PCR, Zieh-Neelsen staining and histopathologic findings in diagnosis of ...

متن کامل

The Value of Bronchoalveolar Lavage in the Diagnosis of Sputum Smear-NegativePulmonary Tuberculosis

Background and Objectives: Sputum smear staining for acid-fast bacilli is initial approach to the diagnosis of pulmonary tuberculosis (PTB) but more than 50% of cases are sputum smear-negative. This study was aimed to investigate the diagnostic value of fiberoptic bronchoscopy (FOB) guided bronchoalveolar lavage (BAL) in patients suspected to have tuberculosis. Methods: This prospective cross-...

متن کامل

Improved sensitivity of sputum smear microscopy after processing specimens with C18-carboxypropylbetaine to detect acid-fast bacilli: a study of United States-bound immigrants from Vietnam.

The goal of this study was to evaluate the effect of the specimen-processing method that uses the detergent C18-carboxypropylbetaine (CB-18) on the sensitivity of acid-fast bacillus (AFB) staining. Vietnamese immigrants with abnormal chest radiographs provided up to three sputum specimens, which were examined for acid-fast bacilli by use of direct auramine and Ziehl-Neelsen staining. The remain...

متن کامل

Comparison of the automated Cobas Amplicor Mycobacterium tuberculosis assay with the conventional methods for direct detection of Mycobacterium tuberculosis complex in respiratory and extrapulmonary specimens.

OBJECTIVE To evaluate the recently available Cobas Amplicor polymerase chain reaction (PCR) system for the detection of Mycobacterium tuberculosis complex (MTBC) in well-characterized clinical specimens and to compare the results with clinical classification, conventional culture and staining techniques. METHODS Three hundred and forty-four clinical specimens consecutively received for cultur...

متن کامل

Utility of acid-fast staining for detection of mycobacteria in cutaneous granulomatous tissue reactions.

OBJECTIVES Ancillary molecular testing on tissue is available for mycobacterial disease; however, judicious use of highly sensitive tests, such as polymerase chain reaction (PCR) and sequencing, should be guided by histologic parameters. We sought to investigate the utility of performing acid-fast stains (AFS) on skin biopsy specimens with granulomatous inflammation without an otherwise obvious...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical pathology

دوره 31 2  شماره 

صفحات  -

تاریخ انتشار 1978